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primary antibodies of chop sab 40744  (Signalway Antibody)
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Signalway Antibody primary antibodies of chop sab 40744
TM treatment-induced Sestrin2 up-regulation and ER stress. (a) mRNA level of Sestrin2 treated with various concentrations of TM (one-way ANOVA, Dunnett t post hoc test; F (5, 18) = 7.62, P < 0.001). (b) Representative western-blot results show Sestrin2 expression and proteins involving ER stress, such as <t>GRP78,</t> CHOP, XBP1s, and XBP1u. (c–f) Western blot quantitative analysis of Sestrin2 and proteins involving ER stress (one-way ANOVA, Dunnett t post hoc test; for Sestrin2: F (5, 6) = 27.27, P < 0.001; for GRP78: F (5, 6) = 8.97, P =0.009; for CHOP: F (5, 6) = 9.12, P =0.009; for XBP1s/XBP1u: F (5, 6) = 20.27, P =0.001). (g) Viability of HepG2 cells with different TM concentrations treatment (one-way ANOVA, Dunnett t post hoc test; F (5, 18) = 291.97, P < 0.001). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.
Primary Antibodies Of Chop Sab 40744, supplied by Signalway Antibody, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TM treatment-induced Sestrin2 up-regulation and ER stress. (a) mRNA level of Sestrin2 treated with various concentrations of TM (one-way ANOVA, Dunnett t post hoc test; F (5, 18) = 7.62, P < 0.001). (b) Representative western-blot results show Sestrin2 expression and proteins involving ER stress, such as GRP78, CHOP, XBP1s, and XBP1u. (c–f) Western blot quantitative analysis of Sestrin2 and proteins involving ER stress (one-way ANOVA, Dunnett t post hoc test; for Sestrin2: F (5, 6) = 27.27, P < 0.001; for GRP78: F (5, 6) = 8.97, P =0.009; for CHOP: F (5, 6) = 9.12, P =0.009; for XBP1s/XBP1u: F (5, 6) = 20.27, P =0.001). (g) Viability of HepG2 cells with different TM concentrations treatment (one-way ANOVA, Dunnett t post hoc test; F (5, 18) = 291.97, P < 0.001). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Journal: International Journal of Endocrinology

Article Title: Sestrin2 Overexpression Ameliorates Endoplasmic Reticulum Stress-Induced Apoptosis via Inhibiting mTOR Pathway in HepG2 Cells

doi: 10.1155/2022/2009753

Figure Lengend Snippet: TM treatment-induced Sestrin2 up-regulation and ER stress. (a) mRNA level of Sestrin2 treated with various concentrations of TM (one-way ANOVA, Dunnett t post hoc test; F (5, 18) = 7.62, P < 0.001). (b) Representative western-blot results show Sestrin2 expression and proteins involving ER stress, such as GRP78, CHOP, XBP1s, and XBP1u. (c–f) Western blot quantitative analysis of Sestrin2 and proteins involving ER stress (one-way ANOVA, Dunnett t post hoc test; for Sestrin2: F (5, 6) = 27.27, P < 0.001; for GRP78: F (5, 6) = 8.97, P =0.009; for CHOP: F (5, 6) = 9.12, P =0.009; for XBP1s/XBP1u: F (5, 6) = 20.27, P =0.001). (g) Viability of HepG2 cells with different TM concentrations treatment (one-way ANOVA, Dunnett t post hoc test; F (5, 18) = 291.97, P < 0.001). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: We bought primary antibodies of GRP78 (Cat#48119), CHOP (SAB 40744) from Signalway Antibody (USA).

Techniques: Western Blot, Expressing

Sestrin2 overexpression ameliorates TM-induced ER stress. (a) Representative western-blot results of Sestrin2 and ER stress-related proteins, ATF6, GRP78, CHOP, XBP1u and XBP1s in the indicated groups. (b) Western blot quantitative analysis of Sestrin2 (unpaired two-tailed t -test; t (4) = −38.44, P < 0.001). (c) Western blot quantitative analysis of ER stress-related proteins (unpaired two-tailed t -test; for ATF6: t (4) = 4.32, P = 0.012; for GRP78: t (4) = 4.54, P =0.010). ∗ P < 0.05, ∗∗∗ P < 0.001.

Journal: International Journal of Endocrinology

Article Title: Sestrin2 Overexpression Ameliorates Endoplasmic Reticulum Stress-Induced Apoptosis via Inhibiting mTOR Pathway in HepG2 Cells

doi: 10.1155/2022/2009753

Figure Lengend Snippet: Sestrin2 overexpression ameliorates TM-induced ER stress. (a) Representative western-blot results of Sestrin2 and ER stress-related proteins, ATF6, GRP78, CHOP, XBP1u and XBP1s in the indicated groups. (b) Western blot quantitative analysis of Sestrin2 (unpaired two-tailed t -test; t (4) = −38.44, P < 0.001). (c) Western blot quantitative analysis of ER stress-related proteins (unpaired two-tailed t -test; for ATF6: t (4) = 4.32, P = 0.012; for GRP78: t (4) = 4.54, P =0.010). ∗ P < 0.05, ∗∗∗ P < 0.001.

Article Snippet: We bought primary antibodies of GRP78 (Cat#48119), CHOP (SAB 40744) from Signalway Antibody (USA).

Techniques: Over Expression, Western Blot, Two Tailed Test